Development of a HPLC method for analysis of DMSP, glycinebetaine and proline in plants
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Development of a HPLC method for analysis of DMSP, glycinebetaine and proline in plants by Fabio Corradini

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Published by University College Dublin in Dublin .
Written in English

Subjects:

  • High performance liquid chromatography.,
  • Dimethylpropiothetin.,
  • Spartina.,
  • Algae.

Book details:

Edition Notes

StatementFabio Corradini.
ContributionsUniversity College Dublin. Department of Botany.
The Physical Object
Pagination(5), 52p. :
Number of Pages52
ID Numbers
Open LibraryOL17495169M

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Therefore, for analysis of these compounds and their metabolites, we have developed a high performance liquid chromatography (HPLC) based method. Results The extraction of PCNB and HCB from soil and minimal salt medium was carried out with ethyl acetate and hexane respectively with good recoveries (98% for PCNB and 97% for HCB).Cited by: 4. Proline, glycinebetaine, oxalic, malic, malonic and citric acid concentrations in the samples were determined using high performance liquid chromatography (HPLC), according to the method . Glycine betaine extraction was measured according to the method of Zhang et al. (), and glycine betaine content was determined by capillary electrophoresis (Model Biofocus , Bio-Rad. An HPLC method using a cation-exchange column and UV detection for the simultaneous determination of dimethylsulphoniopropionate (DMSP), glycinebetaine, prolinebetaine, proline and arginine in.

The aim of this study was to develop a simple high-performance liquid chromatography with standard ultraviolet spectrometric detection (HPLC-UV) method for betaine (N,N,N-trimethylglycine. solutions for HPLC methods. The calibration range was µg/ml, µg/ml and µg/ml Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride of Chlorpheniramine Maleate, respectively in the HPLC methods of analysis for three drugs. Precision and accuracy The extent to which intermediate precision should be. Free proline content can increase upon exposure of plants to drought, salinity, cold, heavy metals, or certain pathogens. Determination of free proline levels is a useful assay to monitor physiological status and to assess stress tolerance of higher plants. Here we describe three methods suitable for determination of free proline content.   1. Introduction. Glycine betaine (N,N,N-trimethylglycine) is a small zwitterionic compound which is produced by a wide variety of organisms (bacteria, plants, invertebrates, and mammals).It was first isolated from the sap of sugar beet, Beta vulgaris, hence the name betaine (Scheibler, ).In mammals, glycine betaine acts as an osmolyte in the inner medulla of the kidney, preserving osmotic.

Development and Validation of RP-HPLC Method for the Simultaneous Determination of Trimethoprim, Sulfadimidine Sodium and Tylosin Tartrate in injectable solution formulation Mashhour Ghanem1, Saleh Abu-Lafi2* 1 Pharmacare Pharmaceutical Company, P.O. Box , Ramallah, Palestine.   X. PHARMACEUTICAL DEVELOPMENT Basic method development has been covered in some earlier texts The evolutionary process of HPLC method development and utilization at each stage of the drug development process to fulfill the scientific, regulatory, and business needs of a pharmaceutical company is described in Chapter Though many HPLC methods have been developed and reported in literature for vitamin analysis for the past two decades, applying certain methods directly from literature more than often fails to reproduce the results reported due to many variables of liquid chromatography. This issue was targeted in this project through the examination of. Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry.